Cultivation and expansion of mesenchymal stem cells from human gingival tissue and periodontal ligament in different culture media

Background: Gingival tissue and periodontal ligament act as sources of mesenchymal stem cells (MSCs) that play a vital role in regeneration, but they both have limitations for cell availability. MSCs cultivated expanded various media formulations could be used basis the development therapy protocols. Purpose: This study aimed to determine optimum culture formulation cultivation expansion human gingival-derived (hGMSCs) (hPDLSCs). Methods: The hGMSCs hPDLSCs were obtained from gingival specimens an adult patient. two different were: 1) α-minimum essential (α-MEM) supplemented with 10% FBS, 100 U/mL penicillin, 100mg/mL streptomycin 2.5 µg/mL amphotericin B; 2) Dulbecco’s minimum media-Low Glucose (DMEM-LG) 2 mMol/L L-glutamine, B. minced-gingival samples seeded 3 cm dishes one experimental media, incubated at 37oC humidified atmosphere 5% CO2. Results: Cell morphology was observed on days five third passage. primary exhibited fibroblast-like morphology, long processes spindle-shaped. grown α-MEM significant increase viability proliferation rate compared DMEM-LG. However, displayed similar types media. Both expressed MSC markers, including CD105, CD146, CD90, did not express CD45. Conclusion: Culture DMEM-LG can hPDLSCs.